LUNA FL 概要

LUNA FL有四种计数模式,是一个功能模块源。配备明场和双荧光光学,允许敏感的检测除细菌以外的大多数细胞类型。LUNA-FL可以从不良碎片中区分原代细胞,如PBMCs、脾细胞、中性粒细胞和干细胞等,以获得准确的细胞计数和活率结果。明场细胞计数、荧光细胞计数、酵母细胞计数和GFP转染检测都在LUNA-FL上完成。这个多功能的细胞计数仪可以方便地放置在细胞培养罩或您的工作台,以适应无缝进入您的工作流程。


产品亮点


几乎适用所有细胞

先进的成像软件和GFP&RFP荧光系统灵敏地检测荧光染色细胞。


二合一计数仪

LUNA-FL™可以在明场和荧光模式下计数,使其成为我们通用的计数器。


计数时间最长30秒

荧光计数:30 s = 采集3张图像+分析;明场计数:10 s = 采集1张图像+分析


GFP转染分析

采集分析高分辨率图像,检测GFP阳性和阴性细胞。

软件使得实验更高效

更低的成本&更大的便利


无论您使用更为便利的一次性精密计数板,还是使用可重复使用计数板片,我们的细胞计数仪都能提供准确和可靠的计数结果。

Cell DB

HL-60 (50% viability)

Experimental condition

HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

HL-60 (high density)

Experimental condition

A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

Jurkat

Experimental condition

Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

Human peripheral blood

Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.

Mouse neuron

Experimental condition

Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

Mouse PBMC

  • Experimental condition

    Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.